m14 cells  (ATCC)

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Identification of differentially expressed genes based on ImmuneScore and StromalScore. ( A-C ) Overall survival analysis of high and low Immune/Stromal/ESTIMATE Scores in patients with metastatic melanoma using the ESTIMATE algorithm. ( D , E ) Heatmap depicting the top 100 DEmRNAs with the most significant p-values between high and low Immune/Stromal Scores (|log Fold change|>1.9, p < 0.001). Blue represents downregulation, while red represents upregulation. ( F ) Venn diagram showing the upregulated and downregulated genes commonly intersecting between Stromal and Immune Scores, with green indicating immune-related DEmRNAs and purple indicating stromal-related DEmRNAs. ( G ) Construction of a protein-protein interaction network for overlapping genes using the STRING online tool (confidence score > 0.900) and Cytoscape software. ( H ) The top 15 genes ranked by nodes in the PPI network are shown in the graph. ( I ) Univariate Cox analysis was conducted on genes with a node count of 15 or more in the PPI network to identify differentially expressed genes significantly associated with prognosis

Article Snippet: Human immortalized epidermal keratinocyte cell line (HaCaT) and human metastatic melanoma cell lines (A2058, M14) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Software

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: The expression of PTPN6 and its correlation analysis with survival. ( A ) Boxplot showing differential expression of PTPN6 in metastatic melanoma samples and normal skin tissue samples. ( B ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with overall survival. ( C ) Correlation analysis of PTPN6 expression in metastatic melanoma samples with progression-free survival. ( D ) RT-qPCR assay analyzed the transcription level of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( E ) Western blotting assay analyzed the expression of PTPN6 in human keratinocyte cell line HaCaT and human metastatic melanoma cell lines A2058 and M14. (** p < 0.01, *** p < 0.001)

Article Snippet: Human immortalized epidermal keratinocyte cell line (HaCaT) and human metastatic melanoma cell lines (A2058, M14) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Quantitative Proteomics, Quantitative RT-PCR, Western Blot

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Overexpression of PTPN6 inhibited the proliferation and viability of metastatic melanoma cells in vitro. ( A–C ) RT-qPCR and Western blotting assays were performed to evaluate the mRNA and protein expression of PTPN6 in A2058 and M14 cells after transfection with PTPN6. The RT-qPCR results showed the relative mRNA expression of PTPN6 normalized to GAPDH. ( D , E ) CCK8 assay was used to determine the impact of PTPN6 overexpression on the proliferation of A2058 and M14 cells. ( F , G ) EdU assay was conducted to assess the impact of PTPN6 overexpression on the viability of A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as the mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Article Snippet: Human immortalized epidermal keratinocyte cell line (HaCaT) and human metastatic melanoma cell lines (A2058, M14) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Over Expression, In Vitro, Quantitative RT-PCR, Western Blot, Expressing, Transfection, CCK-8 Assay, EdU Assay, Standard Deviation

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Overexpression of PTPN6 suppressed the migration and invasion of metastatic melanoma cells in vitro. ( A , B ) Wound healing assay was performed to determine the impact of PTPN6 overexpression on the migration of A2058 and M14 cells. ( C , D ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 overexpression on the migration and invasion of A2058 and M14 cells. ( E , F ) Western blotting assay was conducted to verify the expression of migration and invasion-related genes, including MMP2, MMP9, E-cadherin, and Vimentin, in control, NC, and PTPN6 overexpression groups in A2058 and M14 cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)

Article Snippet: Human immortalized epidermal keratinocyte cell line (HaCaT) and human metastatic melanoma cell lines (A2058, M14) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Over Expression, Migration, In Vitro, Wound Healing Assay, Western Blot, Expressing, Control, Standard Deviation

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Overexpression of PTPN6 inhibited the activity of the JAK2-STAT3 signaling pathway in metastatic melanoma cells. ( A-D ) Western blotting assay was used to assess the expression of p-JAK2, JAK2, p-STAT3, and STAT3 after transfection with the PTPN6 overexpression plasmid. (**** p < 0.0001)

Article Snippet: Human immortalized epidermal keratinocyte cell line (HaCaT) and human metastatic melanoma cell lines (A2058, M14) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Over Expression, Activity Assay, Western Blot, Expressing, Transfection, Plasmid Preparation

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: PTPN6 may affect immune cell infiltration and immune therapy response in metastatic melanoma. ( A ) TIMER 2.0 online analysis tool was used to examine the relationship between PTPN6 and the levels of infiltration of 14 immune cells and tumor purity in metastatic melanoma. ( B ) The correlation between PTPN6 and immune checkpoint therapy (alone or in combination with PD-1 or CTLA-4) response was analyzed using the TCIA database. ( C , D ) Western blotting assay was used to detect PD-L1 expression after PTPN6 overexpression (* p < 0.05, ** p < 0.01)

Article Snippet: Human immortalized epidermal keratinocyte cell line (HaCaT) and human metastatic melanoma cell lines (A2058, M14) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Western Blot, Expressing, Over Expression

Journal: Cancer Cell International

Article Title: Prognostic value and immune infiltration of a tumor microenvironment-related PTPN6 in metastatic melanoma

doi: 10.1186/s12935-024-03625-6

Figure Lengend Snippet: Knockdown of PTPN6 promoted the proliferation, migration, and invasion of metastatic melanoma cells in vitro. ( A , B ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cells after transfection with GV341-PTPN6-3FLAG-SV40-puromycin lentivirus. ( C , D ) Western blotting assay was performed to evaluate the protein expression of PTPN6 in A2058 and M14 cell lines stably overexpressing PTPN6 after transiently transfected siPTPN6 or siNC. ( E , F ) CCK8 assay was used to determine the impact of PTPN6 knockdown on the proliferation of cells. ( G , H ) Wound healing assay was performed to determine the impact of PTPN6 knockdown on the migration of cells. ( I , J ) Transwell migration and Matrigel invasion assays were used to assess the impact of PTPN6 knockdown on the migration and invasion of cells. Representative images were randomly selected from three independent experiments. Data are presented as mean ± standard deviation (SD). (** p < 0.01, *** p < 0.001, **** p < 0.0001)

Article Snippet: Human immortalized epidermal keratinocyte cell line (HaCaT) and human metastatic melanoma cell lines (A2058, M14) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Knockdown, Migration, In Vitro, Western Blot, Expressing, Transfection, Stable Transfection, CCK-8 Assay, Wound Healing Assay, Standard Deviation

Journal: Nanomaterials

Article Title: Parenteral Nanoemulsions Loaded with Combined Immuno- and Chemo-Therapy for Melanoma Treatment

doi: 10.3390/nano12234233

Figure Lengend Snippet: MTT assay performed after 72 h of incubation with the formulations under study. B16 cells: upper left panel; D4M cells: upper right panel; M14 cells: lower left panel; A2058 cells: lower right panel. Abbreviations: IL: Intralipid ® 10%; SOR: sorafenib; TMZ: temozolomide; MIX: drug combination (temozolomide dodecyl ester, sorafenib, ICOS-Fc). Concentrations employed: SOR A = 16 μM; B = 10 μM; C = 8 μM; D = 4.5 μM; E = 0.8 μM. TMZ A = 48 μM; B = 32 μM; C = 24 μM; D = 16 μM; E = 2.4 μM. Statistical analysis: ^ p < 0.05 SOR vs. IL SOR; * p < 0.05 TMZ vs. IL TMZ; § p < 0.05 MIX vs. IL MIX.

Article Snippet: A2058 and M14 human melanoma cells and B16-F10 murine melanoma cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: MTT Assay, Incubation

Journal: Nanomaterials

Article Title: Parenteral Nanoemulsions Loaded with Combined Immuno- and Chemo-Therapy for Melanoma Treatment

doi: 10.3390/nano12234233

Figure Lengend Snippet: Clonogenic assay with B16 (upper left panel), D4M (upper right panel), M14 (lower left panel), and A2058 (lower right panel) melanoma cells. Abbreviations: IL: Intralipid ® 10%; SOR: sorafenib; TMZ: temozolomide; MIX: drug combination (temozolomide dodecyl ester, sorafenib, ICOS-Fc). Cells were treated with the formulations under study for 3 h. Afterwards, the medium was changed, and cells were cultured in drug-free medium for an additional 7 days. Concentrations employed: SOR A = 16 μM; B = 10 μM; C = 8 μM; D = 4.5 μM; E = 0.8 μM. TMZ A = 48 μM; B = 32 μM; C = 24 μM; D = 16 μM; E = 2.4 μM. Statistical analysis: ^ p < 0.05 SOR vs. IL SOR; * p < 0.05 TMZ vs. IL TMZ; § p < 0.05 MIX vs. IL MIX.

Article Snippet: A2058 and M14 human melanoma cells and B16-F10 murine melanoma cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Clonogenic Assay, Cell Culture

Journal: Nanomaterials

Article Title: Parenteral Nanoemulsions Loaded with Combined Immuno- and Chemo-Therapy for Melanoma Treatment

doi: 10.3390/nano12234233

Figure Lengend Snippet: Hypothesized sorafenib (SOR) mechanism of action: ( a ) SOR release profile in culture medium, and ( b ) SOR internalization migration assay (B16, M14 melanoma cells): *** p < 0.005, * p < 0.05, # p < 0.01. ( c ) Hypothesized cellular pathways. Abbreviations: FCS: fetal calf serum; IL: Intralipid ® 10%; RAF: rapidly accelerated fibrosarcoma kinases; RTK: receptor tyrosine kinases.

Article Snippet: A2058 and M14 human melanoma cells and B16-F10 murine melanoma cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Migration